Selection of 5-Methyltryptophan-Resistant Adenine Auxotrophic Cell Line of Datura innoxia MILL
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چکیده
منابع مشابه
Expression of 5-Methyltryptophan Resistance in Plants Regenerated from Resistant Cell Lines of Datura innoxia.
Seventy-nine 5-methyltryptophan-resistant cell lines have been selected from haploid Datura innoxia Mill. cell cultures by plating suspensions in agar medium containing a growth inhibitory concentration of 5-methyltryptophan. Mutagen treatment increased the frequency of resistance. The eleven variants tested posses an altered anthranilate synthase less sensitive to feedback inhibition by trypto...
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Datura innoxia cells from suspension cultures were selected for their ability to grow and divide rapidly in normally lethal concentrations of cadmium. Cells resistant to 12.5, 25, 50, 100, 160, 200, and 250 micromolar cadmium chloride were isolated and utilized to initiate cell suspension cultures resistant to this toxic metal ion. Variant cell lines retained their ability to grow in cadmium af...
متن کامل5-Methyltryptophan resistant cells of Catharanthus roseus.
Several cell lines resistant to 5-methyltryptophan were selected from wild type cells of different Catharanthus roseus suspension cultures. The resistant cells had up to 30 times the normal levels of free tryptophan. Despite the increased pool size of tryptophan anthranilate synthetase activity of resistant cells was as sensitive to inhibition by L-tryptophan as wild type cells. The overproduct...
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Cells resistant to the sulfonylurea herbicides chlorsulfuron and sulfometuron methyl were isolated from a predominantly haploid cell suspension culture of Datura innoxia P. Mill. Exponentially growing cell colonies (aggregates of about 40 cells) were mutagenized with ethyl methane sulfonate, subcultured for 10 days to allow growth recovery and plated on a medium containing either chlorsulfuron ...
متن کاملInvestigation on primary and secondary metabolism of Datura innoxia Mill. tissue cultures
in journal: 1, László I., Szoke É. and Tyihák E. (1998) Effect of Magnesium on the Methylation of Alkaloids in Datura innoxia Mill. Tissue Cultures. Magnesium Research, 11, 236-237.
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ژورنال
عنوان ژورنال: Plant tissue culture letters
سال: 1987
ISSN: 1884-7706,0289-5773
DOI: 10.5511/plantbiotechnology1984.4.90